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Impact of the diet in the gut microbiota after an inter-species microbial transplantation in fish

Under current experimental conditions, Atlantic salmon were used as donors of intestinal microbiota, whereas gilthead seabream were chosen as recipients. In particular, Atlantic salmon with an initial body weight (BWi) of 55.0 ± 0.1 g (mean ± SD) were purchased from SARL SALMO (Gonneville-Le Theil, France), while gilthead seabream with BWi = 100.2 ± 0.9 g were obtained from Niordseas S.L. (Villajoyosa, Spain), and both were transported by road to the facilities of IRTA, La Ràpita (Tarragona, Spain). Atlantic salmon parrs were smoltified as described in Salomón et al.23. Then, salmon smolts were placed in 2000 L-tanks connected to a water recirculation system (IRTAmar™, Spain) and maintained at a water temperature, pH (pH meter 507, Crison Instruments, Barcelona, Spain) and dissolved oxygen (OXI330, Crison Instruments) of 12.1 ± 0.2 °C, 7.4 ± 0.3, and 9.5 ± 0.2 mg/L respectively, under natural photoperiod (8 h light: 16 h darkness) until the beginning of the experiment. Gilthead seabreams were placed in 2000 L-tanks connected to an IRTAmar™ system and water quality parameters were kept at 19.9 ± 2.3 °C, 7.6 ± 0.4, and 6.3 ± 0.6 mg/L. During this period, fish were fed ad libitum with two different feeds; in particular, Atlantic salmon were fed with an experimental diet containing 40% crude protein, 22% crude fat, and 21.6 MJ/kg digestible energy (2–3 mm pellet size; salmon diet)23, whereas gilthead seabream were fed with an experimental compound feed (3–5 mm pellet size) containing 44% crude protein, 20% lipids, and 18 MJ/kg digestible energy (GSB diet).

Ruiz, A., Gisbert, E. & Andree, K.B. Impact of the diet in the gut microbiota after an inter-species microbial transplantation in fish. Sci Rep 14, 4007 (2024). https://doi.org/10.1038/s41598-024-54519-6 

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